伊鸣研究员
Ming Yi Ph.D.
Principal Investigator
Laboratory for Cognitive Neuroscience
Neuroscience Research Institute
Peking University
38 Xueyuan Road
Beijing 100083, China
Tel: + 86(0)10 8280 5083
Email: mingyi@hsc.pku.edu.cn
Academic experience
Dr. Yi received his medical degree in Peking University Health Science Center, and Ph.D. training in John O’Keefe (2014 Nobel laureate) group in the Department of Anatomy and Developmental Biology of University College London (UCL). Dr. Yi is currently a principal investigator and leads the Laboratory for Cognitive Neuroscience in Neuroscience Research Institute of Peking University.
Research: Neuronal basis and functional reconstruction of the memory system
Learning and memory are crucial cognitive functions for daily life. On the other hand, pathological memories for exteroceptive and interoceptive stimuli underlie a variety of neurological diseases such as chronic pain (for nociception), port-traumatic stress disorder (for negative affect) and addiction (for abused substances). Memory traces are encoded and stored by subpopulations of interconnected engram cells widely distributed in the brain.
Yi’s lab, with combinative application of in vivo and in vitro electrophysiology, behavioral testing, opto- and chemo-genetics and molecular biology, focuses on the fascinating and challenging work to understand the neuronal basis of memory and its dysfunction under pathological conditions, to enhance memory functions in animals and humans, and to functionally reconstruct the memory system impaired in various neurological disorders. Our previous work has revealed how hippocampal networks undergo progressive functional deterioration in Alzheimer’s disease (PNAS. 2008) and chronic pain (J Neurosci. 2017; Pain. 2018; Cell Rep. 2019; Cell Rep. 2023), and how these electrophysiological changes correlate with behavioural and pathological alterations. Recently, the lab has achieved erasure of specific fear (Sci Adv. 2020) and pain (Cell Rep. 2022) memory traces from rat neocortices with genetic tools. Ongoing work is focused on functional reconstruction of memory functions through traditional pharmacological methods and novel neural modulation and engineering strategies.
Representative publications
Shao S, Zheng Y, Fu Z, Wang J, Zhang Y, Wang C, Qi X, Gong T, Ma L, Lin X, Yu H, Wan Y, Zhang H, Yi M. Ventral hippocampal CA1 modulates pain behaviors in mice with peripheral inflammation. Cell Rep. 2023; 42(1): 112017.
Qi X, Cui K, Zhang Y, Wang L, Tong J, Sun W, Shao S, Wang J, Wang C, Sun X, Xiao L, Xi K, Cui S, Liu F, Ma L, Zheng J, Yi M, Wan Y. A nociceptive neuronal ensemble in dorsomedial prefrontal cortex underlies pain chronicity. Cell Rep. 2022; 41(11):111833.
Sun H, Fu S, Yin X, Sun X, Qi X, Cui K, Wang J, Ma L, Liu FY, Cui S, Liao FF, Wang XH, Yi M, Wan Y. Development of CRISPR-SaCas9 system for projection- and function-specific gene editing in the rat brain. Sci Adv. 2020; 6: eaay6687.
Ma L, Yue L, Zhang Y, Wang Y, Han B, Cui S, Liu FY, Wan Y, Yi M. Spontaneous pain disrupts ventral hippocampal CA1-infralimbic cortex connectivity and modulates pain progression in rats with peripheral inflammation. Cell Rep. 2019;29:1579–1593.
Ma L, Zhang Y, Yue L, Zhang X, Cui S, Liu F, Wan Y, Yi M. Anterior cingulate cortex modulates the affective-motivative dimension of hyperosmolality-induced thirst. J Physiol. 2019; 597(18):4851-4860.
Jiang Y, Shao S, Zhang Y, Zheng J, Chen X, Cui S, Liu FY, Wan Y, Yi M. Neural pathways in medial septal cholinergic modulation of chronic pain: distinct contribution of anterior cingulate cortex and ventral hippocampus. Pain. 2018. 159(8):1550-1561.
Peng WW, Xia XL, Yi M, Huang G, Zhang ZG, Iannetti GD, Hu L. Brain oscillations reflecting pain-related behavior in freely moving rats. Pain. 2018. 159(1):106–118.
Fan XC, Fu S, Liu FY, Cui S, Yi M, Wan Y. Hypersensitivity of prelimbic cortex neurons contributes to aggravated nociceptive responses in rats with experience of chronic inflammatory pain. Front Mol Neurosci. 2018. 11:85.
Zheng J, Jiang YY, Xu LC, Ma LY, Liu FY, Cui S, Cai J, Liao FF, Wan Y, Yi M. Adult hippocampal neurogenesis along the dorsoventral axis contributes differentially to environmental enrichment combined with voluntary exercise in alleviating chronic inflammatory pain in mice. J Neurosci. 2017; 37(15):4145-4157.
Liu Y, Lai S, Ma W, Ke W, Zhang C, Liu S, Zhang Y, Pei F, Li S, Yi M, Shu Y, Shang Y, Liang J, Huang Z. CDYL suppresses epileptogenesis in mice through repression of axonal Nav1.6 sodium channel expression. Nat Commun. 2017;8(1):355.
Xiao X, Zhao XT, Xu LC, Yue LP, Liu FY, Cai J, Liao FF, Kong JG, Xing GG, Yi M, Wan Y. Shp-1 dephosphorylates TRPV1 in dorsal root ganglion neurons and alleviates CFA-induced inflammatory pain in rats. Pain. 2015; 156(4): 597-608.
Yi M, Zhang H. Nociceptive memory in the brain: cortical mechanisms of chronic pain. J Neurosci. 2011. 31:13343-13345.
Cacucci F *, Yi M *, Wills TJ, Chapman P, O'Keefe J. Place cell firing correlates with memory deficits and amyloid plaque burden in Tg2576 Alzheimer mouse model. Proc Natl Acad Sci U S A. 2008; 105(22): 7863-8.
Funding
- 2023 National Natural Science Foundation (32271053)
- 2023 Beijing Natural Science Foundation (L222016)
- 2019 National Natural Science Foundation (31872774)
- 2018 Beijing Natural Science Foundation (5182013)
- 2015 National Basic Research Program of MOST(2015CB554503)
- 2014National Basic Research Program of MOST(2014CB548200)
- 2012 National Natural Science Foundation (31200835)
Teaching
- “Cognitive functions of the brain”, in Advanced Neurobiology
- “In vivo electrophysiology and optogenetics”, in Research Methods in Neurobiology
- “Advances in hippocampal physiology and function”, in Advances in Neuroscience
- “Neurobiology”, in Foundation of Scientific Research
伊鸣 博士
认知神经科学实验室
联系方式：
地址：北京市海淀区学院路38号北京大学神经科学研究所
邮编：100191
电话：82805083
E-mail: mingyi@bjmu.edu.cn
学术经历：
于北京大学医学部临床医学专业获医学学士学位，英国伦敦大学学院（University College London, UCL）解剖与发育生物学系神经科学专业获哲学博士学位（导师John O’Keefe教授，英国皇家科学院、皇家医学院、美国科学院院士，2014年诺贝尔生理学或医学奖获得者）。现任北京大学神经科学研究所认知神经科学实验室研究员。
研究方向：记忆的精准操控与功能重建
学习与记忆是重要的认知功能。而病理性记忆是慢性痛、创伤后应激综合征、物质成瘾等多种神经系统疾病的共有特征。记忆痕迹由广泛分布在海马、新皮层等脑区的印迹细胞群编码并储存。我们联合应用清醒动物在体电生理记录、光/化学遗传学、分子生物学、脑片膜片钳、行为学等多项先进技术，关注记忆与病理性记忆的细胞基础、动物与人记忆功能增强与疾病状态下的记忆功能重建。我们的工作揭示了阿尔茨海默病（PNAS. 2008）及慢性痛（J Neurosci. 2017; Pain. 2018; Cell Rep. 2019; Cell Rep. 2023）模型中海马神经网络的电生理特征及其行为相关性。近年来，我们应用遗传学技术实现了通路特异性与功能特异性神经元亚群的基因编辑与功能调控，进而实现新皮层中特定恐惧记忆（Sci Adv. 2020）与痛记忆（Cell Rep. 2022）的精确操控。目前实验室的工作集中于应用药物学、神经调控与神经工程等技术，实现疾病状态下的记忆功能重建，为多种以记忆障碍为特征的疾病治疗提供新思路。
核心技术
自由活动动物在体电生理记录与神经计算
通过手术将微电极阵列埋置在动物特定脑区后，在动物清醒自由活动状态下进行行为学实验，同时记录各脑区的神经元动作电位和神经元群局部场电位。进而通过数学方法分析记录到的电信号频率、幅度、相位等特征，在神经网络层面研究脑功能特征。这项技术可以直接、长期、同步观察清醒动物特定核团神经元活动与行为相关性。
光/化学遗传学
光遗传学与化学遗传学技术是神经科学前沿技术，通过光脉冲或特定药物精确调控特定脑区特定类型神经元活动模式，进而研究其对行为的影响。
光遗传学技术首先通过转基因技术或病毒转染，使动物特定脑区特定类型神经元或神经回路表达兴奋性或抑制性光敏蛋白，进而通过光纤用激光或LED光脉冲人为地时空特异性控制光敏蛋白传输兴奋性的阳离子流或者抑制性的阴离子流，进而精确控制细胞或神经回路的活动状态。
化学遗传学技术通过转基因技术或病毒转染，使动物特定脑区特定类型神经元表达专门设计的兴奋性或抑制性G蛋白偶联受体，进而局部或系统注射专门设计的激活此受体的药物控制相应细胞或神经回路的活动状态。其操作比光遗传学更简单，作用更持久。
此外，基因编辑、分子生物学、钙成像、脑片膜片钳、动物行为学、高密度脑电等其他先进技术均有应用。
代表性论文
Shao S, Zheng Y, Fu Z, Wang J, Zhang Y, Wang C, Qi X, Gong T, Ma L, Lin X, Yu H, Wan Y, Zhang H, Yi M. Ventral hippocampal CA1 modulates pain behaviors in mice with peripheral inflammation. Cell Rep. 2023; 42(1): 112017.
Qi X, Cui K, Zhang Y, Wang L, Tong J, Sun W, Shao S, Wang J, Wang C, Sun X, Xiao L, Xi K, Cui S, Liu F, Ma L, Zheng J, Yi M, Wan Y. A nociceptive neuronal ensemble in dorsomedial prefrontal cortex underlies pain chronicity. Cell Rep. 2022;41(11):111833.
Sun H, Fu S, Yin X, Sun X, Qi X, Cui K, Wang J, Ma L, Liu FY, Cui S, Liao FF, Wang XH, Yi M, Wan Y. Development of CRISPR-SaCas9 system for projection- and function-specific gene editing in the rat brain. Sci Adv. 2020; 6: eaay6687.
Ma L, Yue L, Zhang Y, Wang Y, Han B, Cui S, Liu FY, Wan Y, Yi M. Spontaneous pain disrupts ventral hippocampal CA1-infralimbic cortex connectivity and modulates pain progression in rats with peripheral inflammation. Cell Rep. 2019;29:1579–1593.
Ma L, Zhang Y, Yue L, Zhang X, Cui S, Liu F, Wan Y, Yi M. Anterior cingulate cortex modulates the affective-motivative dimension of hyperosmolality-induced thirst. J Physiol. 2019; 597(18):4851-4860.
Jiang Y, Shao S, Zhang Y, Zheng J, Chen X, Cui S, Liu FY, Wan Y, Yi M. Neural pathways in medial septal cholinergic modulation of chronic pain: distinct contribution of anterior cingulate cortex and ventral hippocampus. Pain. 2018. 159(8):1550-1561.
Peng WW, Xia XL, Yi M, Huang G, Zhang ZG, Iannetti GD, Hu L. Brain oscillations reflecting pain-related behavior in freely moving rats. Pain. 2018. 159(1):106–118.
Fan XC, Fu S, Liu FY, Cui S, Yi M, Wan Y. Hypersensitivity of prelimbic cortex neurons contributes to aggravated nociceptive responses in rats with experience of chronic inflammatory pain. Front Mol Neurosci. 2018. 11:85.
Zheng J, Jiang YY, Xu LC, Ma LY, Liu FY, Cui S, Cai J, Liao FF, Wan Y, Yi M. Adult hippocampal neurogenesis along the dorsoventral axis contributes differentially to environmental enrichment combined with voluntary exercise in alleviating chronic inflammatory pain in mice. J Neurosci. 2017; 37(15):4145-4157.
Liu Y, Lai S, Ma W, Ke W, Zhang C, Liu S, Zhang Y, Pei F, Li S, Yi M, Shu Y, Shang Y, Liang J, Huang Z. CDYL suppresses epileptogenesis in mice through repression of axonal Nav1.6 sodium channel expression. Nat Commun. 2017;8(1):355.
Xiao X, Zhao XT, Xu LC, Yue LP, Liu FY, Cai J, Liao FF, Kong JG, Xing GG, Yi M, Wan Y. Shp-1 dephosphorylates TRPV1 in dorsal root ganglion neurons and alleviates CFA-induced inflammatory pain in rats. Pain. 2015; 156(4): 597-608.
Yi M, Zhang H. Nociceptive memory in the brain: cortical mechanisms of chronic pain. J Neurosci. 2011. 31:13343-13345.
Cacucci F *, Yi M *, Wills TJ, Chapman P, O'Keefe J. Place cell firing correlates with memory deficits and amyloid plaque burden in Tg2576 Alzheimer mouse model. Proc Natl Acad Sci U S A. 2008; 105(22): 7863-8.
科研资助与奖励
2023年国家自然科学基金（32271053）
2023北京市自然科学基金-海淀原始创新联合基金（L222016）
2019年国家自然科学基金（31872774）
2019年北京市青年人才托举计划
2018年北京自然科学基金（5182013）
2015年国家重点基础研究发展计划（973计划）（2015CB554503）
2014年973计划青年科学家专题（2014CB548200）
2012年国家自然科学基金（31200835）
2015年国家科学技术进步奖二等奖（5/10）
教学
本科生课程《科研思维训练》：神经生物学
研究生课程《高级神经生物学》：脑的高级认知功能
研究生课程《神经科学进展》：认知神经科学研究进展
研究生课程《神经生物学实验》：清醒动物在体电生理记录与光遗传学技术
北京大学医学部教师教学发展中心培训导师
2017年北京大学医学部教学优秀奖
2014年中华医学会医学教育分会第四届医学（医药）院校青年教师教学基本功比赛特等奖，最受学生欢迎奖，最佳教案奖
2013年北京高校第八届青年教师教学基本功比赛一等奖，最受学生欢迎奖，最佳演示奖
2013年北京大学医学部教学优秀奖
2012年北京大学第十二届青年教师教学演示竞赛（医科类）一等奖
2012年北京大学基础医学院第十二届青年教师教学演示竞赛一等奖